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5ml DNA Fragments Labeling PCR Kits With Dye Lab Research Use 0
Product Descprition
This product includes Foregene's unique new generation of Taq——Foregene Taq Hero, optimized dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The 2× PCR HeroTM Mix system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.
The product is pre-mixed with Electrophoresis Loading Buffer, and after PCR is completed, the sample can be directly loaded for electrophoresis; the operation is more convenient and easier.
Specifications:
DP-20032-B:2× PCR EasyTM Mix(dye) 5ml(1.7ml × 3)
DP-20033-B:2× PCR EasyTM Mix(dye) 10ml(1.7ml × 6)
DP-20033-B:2× PCR EasyTM Mix(dye) 40ml(1.7ml × 24)
Product components:
2× PCR EasyTM Mix(dye) include:0.1U Foregene Taq DNA Polymerase/μl,400 μM dNTPs,20 mM Tris-HCl(pH8.8),100 mM KCl, 3 mM MgCl2, other stabilizers and enhancers,DNA Loading Buffer.
Features&advantages:
The 2× PCR HeroTM Mix system has higher tolerance to PCR inhibitors than the ordinary PCR Mix system, and can easily cope with PCR amplification of various complex templates. The unique reaction system and high-efficiency Taq Hero make the PCR reaction have higher amplification efficiency, specificity and sensitivity.
Kit Application:
-PCR amplification of DNA fragments
-DNA labeling
-DNA sequencing
-PCR plus A tail
Practical examples:
1.Reaction System
2× PCR EasyTM Mix(dye) 10μl
Template DNA xμl
Primer 1(10μM) 0.4μl
Primer 2(10μM) 0.4μl
ddH2O Fill to 20μl
Total volume
20µl
2.Reaction conditions
Temperature Time Cycles
94°C 3min 1
94°C 10sec
25-40
55°C 20sec
72°C 2kb/min
72°C 5min 1
Storage and Shelf life:
It can be stored for a long time at -20°C; for frequent use, it can be stored at 4°C for a short time.